The Brugia Database was generated by aligning 11481 Brugia
sequences contained in dbEST as of February 5, 1998. These sequences were first
sorted by length, PolyA tracts trimmed and regions from both the 5' and
the 3' end containing greater than 25% N's were removed. Sequences of less than 20 bp were eliminated
resulting in 11481 sequences. These sequences were aligned using the cap2
program (Xiaoqiu Huang, 1996, Genomics 33, 21-31). This output file
was then parsed to generate the files containing a library of consensus
sequences and the clustering
The Brugia consensus database contains 4509 consensus sequences, 3433 of which are singletons. 11 different cDNA libraries were used as templates for sequencing.
A number of analyses were done to attempt to assign relatedness or function to the consensus sequences. These analyses are presented here in text form. The files are quite large and are better accessed by using a text search or via the individual sequences which are linked from within the database when information is available.
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Contents by Brian Brunk.