Welcome to ApiDots ApiDots is a database integrating mRNA/EST sequences from numerous Apicomplexan parasites. ESTs and mRNAs were clustered and further assembled to generate consensus sequences. These consensus sequences were then subjected to database searches against protein sequences and protein domain sequences. The underlying relational structure of this database allows researchers to analyze these data and pose biologically interesting questions. Note: ApiDots is currently unavailable, while we complete the move to a new web server. We apologize for the invconvenience. Using ApiDots There are several ways to get started with ApiDots: Click on BLAST at the top of the page and enter your sequence in the form provided. All the genes with significant sequence similarity to your query sequence will be displayed. Click on query at the top of the page to retrieve sequences of interest by querying the database according to a number of different criteria,including keyword search, dbEST library, accession number, etc. Click on boolean at the top of the page to combine several queries in a variety of ways using "AND"/"OR" boolean operation. The history page allows viewing previous query result and further "UNION"/"INTERSECT"/"SUBTRACT" selected query results. Supplemental Results Most abundant genes Stage-specific gene expression Apicomplexa-specific genes Reference Li, L., B.P. Brunk, J.C. Kissinger, D. Pape, P. Tang, R. Cole, J. Martin, T. Wylie, M. Dante, S.J. Fogarty, D.K. Howe, P. Liberator, C. Diaz, J. Anderson, M. White, M.E. Jerone, E.A. Johnson, J.A. Radke, C.J. Stoeckert Jr., R.H. Waterson, S.W. Clifton, D.S. Roos and L.D. Sibley. 2003. Gene discovery in the Apicomplexa as revealed by EST sequencing and assembly of a comparative gene database. Genome Res. 13(3):443-54.
GUS database and interfaces Copyright (2000-2007) Computational Biology and Informatics Laboratory Comments/questions: apidots@pcbi.upenn.edu